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A standard stock solution of brimonidine tartrate (1 mg/mL) was prepared using the mobile phase.
Cordycepin and adenosine were separated by gradient elution using the mobile phase with the composition of water methanol (95.0/5.0 to 58.4/41.6 in 20 min, v/v).
Fraction Fr-3-2 were further purified by HPLC using the mobile phase of 33%CH3CN/H2OO to give compounds 8 (18 mg) and 6 (16.9 mg).
Fr-IV was purified by silica gel column using the mobile phase of CH3Cl/CH3OH (3:1, v/v) to afford compound 17 (162 mg).
Standard calibration solutions (5 to 1,000??g?mL?1) for the assessment of linearity were prepared from this stock solution using the mobile phase.
Separation method of ibuprofen, phenylephrine HCl, and cetirizine from soft gels was reported using the mobile phase of acetonitrile and hexanosulphonate (Cosmes and Rodriguez [2012]).
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An isocratic flow method with 0.3 mL/min was used; the mobile phase consisted of 98%% (v/v) methanol (0.2 % v/v ammonium formate) and 2%% (v/v) acetic acid (0.2 M, adjusted to pH 6 with ammonia).
Prior to use the mobile phase was filtered through 0.45 mm Millipore membrane filters and degassed by sonication in an ultrasonic bath.
The amounts of fructose, glucose, and sucrose were measured using a High Performance Carbohydrate Column (Waters) at 35°C, and 80% acetonitrile was used as the mobile phase.
A 10 mM NaOH solution was used as the mobile phase (flow rate 1 mL/min) and glucose and cellobiose were used as standards.
Silica with a 60~230 mesh was used as the stationary phase and organic solvents (hexane, dichloromethane, ethyl acetate, and methanol) were used as the mobile phase.
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