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After the allotted treatment times, total RNA was extracted from H358 cells using the miRNeasy protocol from Qiagen.
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RNA was extracted from all dissected tissues using the miRNeasy Mini Kit protocol and the automated QIAcube instrument (QIAGEN).
Furthermore, total RNA was isolated from frozen brain, heart and liver tissues using the miRNeasy Mini Kit protocol (Qiagen, Canada) with no modifications.
Quantitative real time PCR Total RNA was isolated from the Leydig cell lysates using the miRNeasy mini kit following the manufacturer's protocol (Qiagen, Shanghai, China).
RNA was extracted using the miRNeasy kit (Qiagen) according to the manufacturers' protocols.
RNA was isolated from plasma using the miRNeasy Serum/Plasma kit (Qiagen) according to manufacturer's protocol.
Total RNA was isolated from dorsal cervical subcutaneous skin tissue using the miRNeasy extraction kit (Qiagen), following the manufacturers standard protocol.
After 24 hours of incubation, the cells were washed once with phosphate buffered saline and total RNA was isolated using the miRNeasy Mini Kit, according to the manufacturer's protocol (QIAGEN).
Total RNA was purified from the tumor samples stored in RNAlater Solution using the miRNeasy kit (Qiagen) according to the manufacturer's protocol once the development of adenomas were validated by the histopathological analysis.
Small RNA species (including miRNA) were also extracted by modifying the PAXgene procedure by harvesting the flow-thorough of the PAXgene RNA column, and isolated using the miRNeasy Mini Kit (Qiagen), according to the manufacturers' protocols.
Total RNA was extracted from cores of FFPE samples using the miRNeasy FFPE kit (Qiagen) according to the manufacturer's protocol.
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