Total RNAs were isolated from the hPGRN-3T3 stable cell line using the miRNeasy mini kit (Qiagen) according to the manufacturer's instruction.
Total RNA from pAGMK cells and the cell lines was extracted and purified using the miRNeasy mini kit according to the manufacturer's procedures (Qiagen Inc., Valencia, CA).
Small RNA was isolated from the hippocampus using the miRNeasy mini kit (Qiagen, Netherlands).
Total RNA was isolated from 60 μl of each plasma sample using the miRNeasy mini kit (Cat no 217004, Qiagen).
Large RNA was extracted from SI crypts using the miRNeasy mini kit and RNeasy MinElute Cleanup kit (QIAGEN).
miRNA was isolated from mouse tissue using the miRNeasy mini kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instruction.
Total RNA was extracted from 1x10 cells using the miRNeasy mini kit (Qiagen) following the manufacturer's recommendations.
RNA was extracted from approximately 50 mg of frozen skeletal muscle and adipose tissues using the miRNeasy mini kit (Qiagen, Valencia, CA, USA) according to the manufacturer's protocol.
Quantitative real time PCR Total RNA was isolated from the Leydig cell lysates using the miRNeasy mini kit following the manufacturer's protocol (Qiagen, Shanghai, China).
Total RNA was extracted using the miRNeasy mini kit (Qiagen), according to the manufacturer's instructions, including treatment with RNase-free DNase (Qiagen).
Total RNA from plasma was extracted using the miRNeasy mini kit (Qiagen, Valencia, CA, USA), and total RNA from cells and tissues was isolated using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA).
