Sentence examples for using the mirneasy column from inspiring English sources
Total RNA was then isolated using the miRNeasy column purification kit.
The RNA was extracted as described above using Qiazol (Qiagen) and prepared for real-time PCR using the miRNeasy columns (no. 217004, Qiagen).
The aqueous phase was removed and mixed with 1.5× volume of ethanol and miRNAs were isolated from the aqueous phase using the miRNeasy silica spin columns (Qiagen) per manufacturer instructions with the addition of one extra wash with the RPE buffer and eluted in 50 μL of nuclease free water and stored at −80°C.
Total RNA was isolated using the miRNeasy kit with in-column DNAse I treatment (Qiagen).
Small RNA species (including miRNA) were also extracted by modifying the PAXgene procedure by harvesting the flow-thorough of the PAXgene RNA column, and isolated using the miRNeasy Mini Kit (Qiagen), according to the manufacturers' protocols.
Total RNA was isolated from frozen embryos or tissue homogenates using the miRNeasy Mini kit (Qiagen) with an on-column DNA purification with RNase Free DNase set (Qiagen).
For purposes of microarray analysis and quantitative RT-PCR, total RNA was prepared using the miRNEasy kit (Qiagen, Valencia, CA) per manufacturer's instructions; on-column DNAse digestion was performed to eliminate contamination with genomic DNA.
Total RNA was isolated using the miRNeasy Mini kit (QIAGEN), and DNA was removed by an on-column digestion of RNase-Free DNase (QIAGEN) during the RNA isolation.
For microarray analysis, RNA of three biological replicates was isolated using the miRNeasy Mini Kit (Qiagen) according to manufacturer's instructions with additional on-column DNase I digestion.
The miRs were extracted with chloroform and the aqueous phase was further processed using the miRNeasy Mini kit (Qiagen, Valencia, CA).
Total RNA, including miRNA from plasma, was isolated using the miRNeasy kit (Qiagen) with minor modifications.
