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By using the minimal fluorescence of a dark-adapted sample (F0) the interdependence between photosynthetic processes and fluorescence is minimized; the fluorescence is influenced by biomass alone (Geel et al. 1997).
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The minimal fluorescence yield (F0) was determined using 15 min dark-adapted samples.
The minimal fluorescence yield (Fo) was measured by using modulated light that was sufficiently low (< 0.1 μmol m-2s-1), and the maximal fluorescence yield (Fm) was determined by a 0.8 s saturating pulse at 8000 μmol m-2s-1 in the dark-adapted leaves.
All the measurements in the dark-adapted state were made after covering the leaves with an aluminium foil for 30 min. The minimal fluorescence (F0) in the dark-adapted state was determined using the measuring modulated light, which was sufficiently low (<0.1 μmol m−2 s−1) not to induce any significant variation in fluorescence.
The minimal fluorescence.
The minimal fluorescence level (F0) in the dark-adapted state was measured when only the LED light was turned on.
The minimal fluorescence level during illumination (F0') was calculated from measured values of F0, Fm, Fm' and Fv [37].
In our analysis, a slight red (soft pink) fluorescence was established as the minimal fluorescence signal.
Under optimal conditions the O step represents the minimal fluorescence intensity (F0) [ 30].
Because of the minimal fluorescence intensity of NBD-PZ in PBS, it was not necessary to wash the colonic mucosa before the fluorescence observation.
The minimal fluorescence level (Fo) in the dark-adapted state was measured when only the LED light was turned on.
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