Sentence examples for using the microsatellite from inspiring English sources
We identified 88,256 simple sequence repeat (SSR) motifs using the MIcroSAtellite program34 in the pearl millet genome sequence and designed primers for 74,891 SSR-containing sequences (Supplementary Tables 23 and 24), which can be used by the pearl millet community for genetics and breeding applications.
Loss of heterozygosity (LOH) was assessed as previously described, using the microsatellite markers D31259 and D3S3701, which flank PPARG [6].
However, the peak using the microsatellite panel was 29cM away from the SNP peak (D4S1591 GATA30B11, LODpat = 2.96, p = 0.008).
The chord distance matrix was built using the Microsatellite analyzer (MSA 4.00) software [68], and principal component analysis was performed under Genalex.
Loss of heterozygosity (LOH) in tumors with mutations in CHEK2 was assessed using the microsatellite marker, D22S275, which maps to intron 4 of CHEK2.
In order to characterize the minke whale species/sub-species using the microsatellite data generated here, population genetic summary statistics, FST values, and potential deviations from Hardy Weinberg equilibrium (HWE) were computed in the programs MSA [27] and Genepop [28].
In addition, heterozygosity was measured using the microsatellite-specific parameter d (Coltman et al. 1999).
Methods and Results: Twenty microsatellite loci were identified in M. spicatum using the microsatellite-enriched library method.
Methods and Results: Using the microsatellite-enriched library and PCR-based screening method, 22 microsatellite markers were developed and 10 showed high polymorphism in a population.
Relationship between the calf and the parents was ensured by genotyping their DNA using the microsatellites BM2113, BM1824, BM1818, ETH10, ETH225, ETH3, INRA012, INRA005, and INRA63 [ 21].
Table S3) were ascertained by using the "Microsatellites" track within the UCSC Genome Browser, which includes cases with at least 15 uninterrupted repeats, and therefore highly likely to be polymorphic.
