Sentence examples for using the microarray hybridization from inspiring English sources
Recent studies using the microarray hybridization method suggest that cuticle-related genes may also be associated with resistance [ 21].
The High-Capacity cDNA Archive Kit (Life Technologies, Foster City, CA) was used to prepare cDNA from total RNA from three of the female donors not used in the microarray hybridization experiments.
Only samples that showed high RNA integrity (RIN >7) were used for the microarray hybridization and quantitative PCR analysis.
In order to increase the reliability of the biological validation, the real time RT-PCR was performed on RNA samples originating from a separate set of animals distinct from the ones used for the microarray hybridization experiments.
From this, 4 μg of DNA was labeled with fluorescent Cy5 dye and used for the microarray hybridization (GE Amersham, USA).
Three biological replicates of MCF-7 J1 clones and two independent siRNA-transfection replicates were used for the microarray hybridization.
Specific primers were designed to perform a qPCR for four selected genes with cDNAs synthesized from the same RNAs used for the microarray hybridization.
The expression levels of selected genes in the microarray data were validated by performing qRT-PCR on the amplified RNA samples that were used for the microarray hybridization.
Numeric data was extracted from the two-channel hybridization images using the microarray image analysis software, BlueFuse (BlueGnome Ltd ,Cambridge).
The total RNA samples (also used for the microarray hybridizations) were quantified using NanoDrop ND-1000 (NanoDrop Technologies, Wilmington, DE) and 500 ng was reverse transcribed for 50 min at 42°C in a 20-µl reaction with 200 U of SuperScript II reverse transcriptase (Invitrogen, Carlsbad, CA) and 0.5 µg of Oligo (dT 12 18 primer following the manufacturer's protocol.
Gene expression of samples from M-ZE and LT-SE were compared using the same RNA extractions used for the microarray hybridizations.
