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Subsequent transcriptional profiling experiments using the microarray constructed from ESTs yielded the transcriptional signatures of the pea SAM, and we have reported a repertoire of transcripts with putative or unknown functions that are differentially regulated in the SAM.
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As this is amenable by comparative genomics, the present study was geared toward detecting C58 gene homologs in other genomes by using a microarray constructed with probes spanning the entire C58 genome.
In the present study, we examine the relationship between hybridization ratio and sequence divergence using a cDNA microarray constructed for D. melanogaster.
Real-time quantitative PCR analysis of this gene set provided data consistent with the quantitative measures by microarray analysis, using the newly constructed B. bronchiseptica oligonucleotide microarray (r2 = 0.94).
Investigators have already analyzed gene expression in Crassostrea virginica and Crassostrea gigas using cDNA microarrays constructed from 6,780 [ 27] and 9,058 [ 21] genes.
Using the newly constructed oligonucleotide B. bronchiseptica microarray, all of these transcripts were found to be positively regulated by BvgAS.
Gene expression studies have previously been performed for genetically less well-known Drosophila species using microarrays constructed for D. melanogaster, but due to high levels of genetic divergence between D. melanogaster and the species under investigation (usually several My), these kinds of data may be difficult to interpret and may lack reliability [e.g. [ 31]].
Briefly, a 70-mer oligonucleotide microarray constructed using the genome sequence of S. Typhimurium strain SL1344 was used for hybridization with the cDNA of each strain.
BRCA1 IHC was done on whole sections, while other IHC markers were assessed using sections from a tissue microarray constructed with two 0.6 mm cores per case.
The microarray used was a 17 K bovine cDNA microarray constructed at ARK Genomics (ArrayExpress accession number A-MEXP1592).
Because the empty construct and untransfected cells could not be discriminated by microarray, we used the empty construct as the control RNA's for qRT PCR.
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