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We searched for the target genes of miRNAs using the miRanda application (Sep. 2008 release) [ 64].
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Predicted miRNA targets were determined using the miRanda algorithm (http://microrna.sanger.ac.uk/targets/v5/) and TargetScan v4.2 (http://www.targetscan.org/).org/
Target prediction was done using the miRanda tool [ 56] with default parameters.
In this study, putative small RNA target sites were searched by using the miRanda software.
Potential targets for miRNAs were predicted using the miRanda, RNAhybrid and TargetScan algorithms simultaneously.
1241 hits were found using the miRanda algorithm [ 54], whereas 353 targets were found using the TargetScan algorithm [ 12].
We further analyzed the topologic property of a similar network derived by using the miRanda prediction data.
Using the miRanda program, we searched potential targeting sites of MIWI-associated piRNAs within the 3′UTRs of MIWI-associated mRNAs.
Each sequence containing the non-reference allele was analyzed for potential miR-target sites using the miRanda software [ 23].
For example, in mirSVR [ 26], the authors use the miRanda algorithm [ 51] to generate the initial candidate set.
One is the miRBase::Targets (release 5) database, which uses the miRanda algorithm to predict miRNA targets [ 6, 31].
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