Sentence examples for using the miRNeasy kit from inspiring English sources
Total RNA was extracted from 80 frozen SAT biopsies using the miRNeasy kit (Qiagen, Venlo, the Netherlands), quantified with a NanoDrop spectrophotometer (Thermo Scientific, Wilmington, DE, USA), and its integrity assayed on an Agilent 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA).
Total RNA, including miRNA from plasma, was isolated using the miRNeasy kit (Qiagen) with minor modifications.
Total RNA was isolated using the miRNeasy kit with in-column DNAse I treatment (Qiagen).
RNA was then isolated using the miRNeasy kit (Qiagen) according to the manufacturer's instructions.
RNA was extracted from mouse primary hippocampal neurons and dissected hippocampi using the miRNeasy Kit (Qiagen) according to the manufacturer's instructions.
For PCR analysis, total RNA was extracted using the miRNeasy Kit (Qiagen, Hilden, Germany) according to the instructions by the manufacturer.
To isolate the RNA including the miRNA from whole blood samples, we used the miRNeasy Kit (Qiagen GmbH, Hilden) as previously described [ 8].
Large RNA was extracted from SI crypts using the miRNeasy mini kit and RNeasy MinElute Cleanup kit (QIAGEN).
Total RNA was isolated from the biopsies using the miRNeasy Mini Kit or the Allprep DNA/RNA/Protein Mini kit (Qiagen, Inc., Valencia, CA) according to manufacturer's protocol.
The validation of the temporal lobe of eight LOAD patients and eight controls was done after extracting the RNA using the miRNeasy Mini Kit (Qiagen, Venlo, The Netherlands).
Total RNA was purified using the miRNeasy Mini kit (Qiagen), the quality control was done with the Agilent Bioanalyzer 2100, using the RNA 6000 nano kit (Agilent).
