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Data processing was performed using the Mev software version 4.3 (Saeed et al. [2006]).
For the developmental stage data, Affymetrix values were log10 transformed, heat maps generated and hierarchical clustering done using the MeV software package (Eisen et al. 1998).
The MALDI-TOF MS data was then clustered hierarchically using the MeV software.
The gene expression patterns in ATF3+/+ and ATF3-/ cells that were treated with Kdo2-Lipid A were visualized using the MeV software.
Cluster analysis was performed using the MeV software from the TM4 microarray software suite using the default settings (Euclidean distance, and a maximum of 50 iterations), using the RMA normalized expression values from all 12 samples (3 replicates for each of the four treatments) [60].
Heat maps were created using the MeV software.
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The data was transformed into log2 scale to build the heat map using the MeV 4.0 software package.
The resulting data were clustered and visualized using the Mev v3.1 software.
Statistical analysis (two-way ANOVA) of the data was done using the MeV (Multi Experiment Viewer) software (Saeed et al., 2003).
The data corresponding to SKP1 genes were extracted (Supplementary Table S2) and analysed using the MeV 4_5_1 software (http://www.tm4.org/mev/).
From these Illumina data, the P450 genes highly expressed in different tissue were hierarchically clustered using the MeV v4.5.1 software.
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