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Analysis of variance and moderated t-test were carried out for finding the differentially expressed exons and genes in each developmental stage, using the methods implemented into the Limma package.
We identified potential gene conversion events within each subfamily using the methods implemented in the GeneConv program [ 51] which identifies identical fragments shared between pairs of nucleotide sequences.
Using the methods implemented in CNA, this model was able to provide deeper insights in the functioning of the signalling network governing T-cell activation and to unravel important and previously unknown aspects of this complicated process.
40 We estimated the baseline survivor function based on zero values of centred continuous variables, with all binary predictor values set to zero, using the methods implemented in Stata.
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We took this point into account by using the method implemented in MERLIN [79].
The functional clustering was performed using the method implemented on the DAVID website http://david.abcc.ncifcrf.gov/.abcc.ncifcrf.gov/
The orthologous protein sequences were aligned using the method implemented in muscle [ 31].
Logistic regression was used to calculate the ORs of the haplotypes using the method implemented in the haplo.ccs package (36).
The ROC statistic was calculated using the method implemented in the commercially available software MedCalc (http://www.medcalc.org).
Maximum-likelihood (ML) phylogenetic trees were estimated for both datasets using the method implemented in the PAUP* package (8 ).
Bayesian inferences were performed on the Phylogeny.fr platform (http://www.phylogeny.fr/) [ 72] using the method implemented in the MrBayes (v3.1.2) program [ 73, 74].
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