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We took this point into account by using the method implemented in MERLIN [79].
The orthologous protein sequences were aligned using the method implemented in muscle [ 31].
The functional clustering was performed using the method implemented on the DAVID website http://david.abcc.ncifcrf.gov/.abcc.ncifcrf.gov/
The ROC statistic was calculated using the method implemented in the commercially available software MedCalc (http://www.medcalc.org).
Logistic regression was used to calculate the ORs of the haplotypes using the method implemented in the haplo.ccs package (36).
Maximum-likelihood (ML) phylogenetic trees were estimated for both datasets using the method implemented in the PAUP* package (8 ).
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Analysis of variance and moderated t-test were carried out for finding the differentially expressed exons and genes in each developmental stage, using the methods implemented into the Limma package.
We identified potential gene conversion events within each subfamily using the methods implemented in the GeneConv program [ 51] which identifies identical fragments shared between pairs of nucleotide sequences.
Using the methods implemented in CNA, this model was able to provide deeper insights in the functioning of the signalling network governing T-cell activation and to unravel important and previously unknown aspects of this complicated process.
40 We estimated the baseline survivor function based on zero values of centred continuous variables, with all binary predictor values set to zero, using the methods implemented in Stata.
To deal with this point, we used the method implemented in Merlin which performs well in our particular case: relatively few parents missing and a low LD between markers.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com