Fluorescence microscopic sample images were taken with a Diagnostic Instruments camera (Model: SPOT 9.0 Monochrome-6, Sterling Heights, MI, USA), acquired using the Metamorph software (version 6.2r4, Molecular Devices, Sunnyvale, CA, USA) and processed with IrfanView (version 3.97, Wiener Neustadt, Austria) and Adobe Photoshop (version CS2) software.
The analysis of the distribution of D1R and D2R-immunopositive asymmetrical synapses was performed on digital images obtained with a computer linked directly to CCD camera on the Tecnai 20 EM Philipss) electron microscope at a final magnification of 2500 to 6000 using the Metamorph software (version 4.6r5, Universal Imaging, Paris, France).
Fluorescence analysis was performed using the Metamorph Program version 7.0.
Fluorescence analysis was performed using the Metamorph program version 7.0 (Molecular Devices, LLC, Sunnyvale, CA, USA).
Images used for calculating plaque sizes were analyzed using the Metamorph Imaging series version 7.0r1 (Universal Imaging, West Chester PA, Molecular Devices Corporation).
Finally, the immunoblots were quantified using the Metamorph software and expressed as a ratio of phosphorylated to total protein or total protein to β-actin.
Length of branches was evaluated using the Metamorph program.
Actin filament lengths were quantified using the Metamorph image software.
Data were analysed using the Metamorph software 7.1.2.0.
The quantification of the co-localization was done using the Metamorph v7.5.6.
