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Finally, the immunoblots were quantified using the Metamorph software and expressed as a ratio of phosphorylated to total protein or total protein to β-actin.
Coated and uncoated beads were prepared and immediately placed on glass slides and optical micrographs were obtained and analyzed using the Metamorph software available with the microscope.
Data were analysed using the Metamorph software 7.1.2.0.
Video images were digitized and analyzed using the Metamorph software (Universal Imaging, Ypsilanti MI).
Images were taken with a digital camera Cool SNAPES (Photometrics) using the MetaMorph software.
Cytotoxicity was evaluated by fluorescent time-lapse microscopy for 48 h using the MetaMorph software (Molecular Devices).
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All vessel morphology analyses where performed using the Metamorph Offline software (Metamorph, Microscopy Automation and Image Analysis Software 2011, Molecular Devices Inc., Sunnyvale, CA, USA).
Actin filament lengths were quantified using the Metamorph image software.
Following digital photography, the colony number was quantified using the MetaMorph imaging software program.
Using the Metamorph imaging software, a 2D image was constructed using the middle plane image from each DG MosaiX stack.
Following digital photography, the total colony area was quantified using the MetaMorph imaging software program (Molecular Devices, Downingtown, PA).
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