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The area of both dorsal and ventral regrowth was measured using the MetaMorph office programme (Molecular Devices Corporation, USA).
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Finally, the immunoblots were quantified using the Metamorph software and expressed as a ratio of phosphorylated to total protein or total protein to β-actin.
Coated and uncoated beads were prepared and immediately placed on glass slides and optical micrographs were obtained and analyzed using the Metamorph software available with the microscope.
Length of branches was evaluated using the Metamorph program.
Actin filament lengths were quantified using the Metamorph image software.
Data were analysed using the Metamorph software 7.1.2.0.
The quantification of the co-localization was done using the Metamorph v7.5.6.
Cytotoxicity was evaluated by fluorescent time-lapse microscopy for 48 h using the MetaMorph software (Molecular Devices).
Following digital photography, the colony number was quantified using the MetaMorph imaging software program.
Monochrome images were analyzed using the metaMorph Imaging System (Universal Imaging Corporation).
Video images were digitized and analyzed using the Metamorph software (Universal Imaging, Ypsilanti MI).
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