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After cDNA isolation and purification using Qiagen PCR purification kit, input material was amplified using the MessageAmp II aRNA Amplification kit.
After extraction, RNA was amplified by using the MessageAmp II aRNA Amplification Kit (Ambion).
Both sample and reference RNAs were amplified using the MessageAmp II aRNA amplification kit (Ambion, Austin, TX).
Total RNA (2 3 ng) was amplified using the MessageAmp II aRNA Amplification Kit (Ambion, USA) according to manufacturer's recommendations.
RT, second strand synthesis, cDNA purification and in vitro transcription with Biotin-11-UTP were performed using the MessageAmp II aRNA Amplification Kit (Ambion).
Only high quality RNA was subjected to two rounds of linear amplification using the MessageAmp II aRNA Amplification Kit (Ambion, USA) according to the manufacturer's instructions and amplified RNA (aRNA) was quantified using the RNA 6000 Pico Assay Kit.
Similar(45)
mRNA was linearly amplified using the MessageAmp II-Bacteria kit (Ambion) according to the manufacturer's instructions.
RNA was amplified using the 'MessageAmp II-Bacteria RNA Amplification Kit' (Ambion) according to the manufacturers' instructions.
200 ng of total RNA were applied for Cy3-labelling reaction using the MessageAmp II-Bacteria Kit according to supplier's recommendation (Ambion; Kaufungen, Germany).
One microgram of total RNA was used to make the biotin-labeled antisense RNA (aRNA) using the MessageAmp II-Biotin Enhanced Single Round aRNA amplification kit (Ambion) following the manufacturer's procedures.
Quantity and quality controls were performed and samples were processed using the MessageAmp II-Biotin Enhanced, Single Round aRNA Amplification Kit (Ambion, Life Technologies, Darmstadt, Germany) as described earlier [ 21, 37].
More suggestions(15)
using the Resplex ii
using the messageamp MessageAmp
using the HiScript® ii
using the ImProm ii
using the Quickchange ii
using the Nuclefector ii
using the QuikChange ii
using the XCell ii
using the MacSynergy ii
using the Rediprime ii
using the messageamp MessageAmp-Biotin
using the Aerophone ii
using the QuickChange ii
using the Rediprime™ ii
using the messageamp MessageAmp™II-Biotin
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