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While this is not the first in vivo demonstration of speckle images at multiple exposure durations [ 17], we note that these images span almost 3 orders of magnitude of exposure duration which is possible with an inexpensive camera using the MESI approach.
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The estimates of relative blood flow measures obtained using the MESI technique were found to be statistically similar in 10 locations across the thin skull.
This indicates that the relative blood flow measures obtained using the MESI technique are unaffected by the presence of the thin skull.
In region 1 we estimated the average percentage reduction in blood flow due to death in all animals to be about 99% using the MESI technique and 92% using the LSCI technique.
The ratio of the correlation time in region 3 to the correlation time in region 4 was found to be 0.883 ± 0.055 using the MESI technique, while this ratio was estimated to be 0.889 ± 0.019 using the LSCI technique.
The estimates of average percentage reduction in blood flow obtained using the MESI technique were found to be statistically greater than those obtained using the LSCI technique with a 5% significance level.
The average percentage reduction in blood flow in the blood vessel, due to the ischemic stroke in all animals was estimated to be 97.3 ±2.09% using the MESI technique and 87.67 ±7.04% using the LSCI technique.
Under baseline conditions, the ratio of the correlation time in region 1 to the correlation time in region 2 was found to be 0.6238 ± 0.0238 using the MESI technique, while this ratio was estimated to be 0.3771 ±0.0215 using the LSCI technique.
Figure 5a shows the relative blood flow change as measured using the MESI technique in region 1, in the same animal as in Figure 4. Since τ c can be assumed to be inversely related to blood flow [ 13], relative blood flow may be defined as the ratio of τ baseline to τ measured.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com