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Because P. falciparun also expresses PKA (PfPKA) we measured the effect of Ang II or Ang-(1 7) on erythrocyte PKA activity using the membrane preparation (Fig. 7).
Similar(59)
The semi-IPN structure was formed during the membrane preparation.
The total concentration of tissue proteins was assayed from the original homogenate spectro-photometrically with bicinchoninic acid (Smith et al. 1985); in addition, we assessed the concentration of membrane proteins from the membrane preparations used for radio-ligand binding.
The total concentration of tissue proteins was assayed spectrophotometrically by dye binding (Smith et al. 1985); in addition, we assessed the concentration of membrane proteins from the membrane preparations used for radioligand binding, as described below.
Table 5 Determination of moxifloxacin in some pharmaceutical preparations using the proposed membrane sensors Preparation Moxifloxacin (nominal,value) Proposed method* R,% (RSD,%) USP R,% (RSD,%) MOX-PM MOX-PT MOX-PT Reconstituted powder 20 mg 98.0(1.6) 98.0 (1.5) 98.5(1.6) 98.5 (1.6) Avelox tablet 400 mg 98.0 (1.7) 98.0(1.6) 98.5(1.7) 99.0 ( 1.8) *Average of five determinations.
Total protein in the membrane preparations was quantified using the bicinchoninic acid assay (BCA) and bovine serum albumin (BSA) as standard and the presence of Abcb4 protein in the membranes was checked in 1 μg of total protein by Western blotting using the anti-MDR1 antibody C219 (Additional file 1: Figure S5) as described previously [ 58].
Many studies [24, 25, 26, 27, 28, 29, 30, 31] can be found in the open literature on the use of polyimides for the membranes preparation; in them, the mass transport proprieties were usually evaluated feeding single gas such as CO2, CH4, N2, H2, O2 and mixtures of CO2/CH4 or CO2/N2 in the temperature range 25 75 °C.
The homogenate was used for membrane preparation as described previously [ 21].
However, most of researches have used solvents that are environmentally unfriendly during the membranes preparation.
For the membranes' preparation Matrimid® 5218 was used as polymer matrix whereas graphene oxide (GO) was used as filler material.
To understand the role of PLD1-μ2 intEGFRtion in endocytosistosis, we first performed in vitro membrane recruitment analysis using membrane preparation of wild type PLD1 and rat brain cytosol.
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