Sentence examples for using the membrane fraction from inspiring English sources

* analyses were performed by Western blotting using the membrane fraction of hmAchR M3 over-expressing Sf9 cells; ** significantly different from the controls (p = 0.043; Wilcoxon test).

The membralin protein data shown in Figure 3A were obtained by using the membrane fraction of protein purified from approximately half of the brain (this is now indicated in the figure legend).

To assess whether PBC patients produce autoantibodies to the hmAchR of the M3 type, we next performed a Western blotting analysis using the membrane fraction of Sf9 cells over-expressing the recombinant hmAchR receptor protein (rhM3R) of the M3 type.

In Figure 7C, the mutants are lower because we used the membrane fraction for the proteinase K protection assay.

After the wash step, we performed the TBSV replication assay for 3 hours at 25°C using the above membrane fraction in combination with the untreated soluble fraction according to [23].

After 30 min incubation at 4°C, the solutions were centrifuged again at 100,000× g for 60 min, and the second supernatant fractions were used as the membrane fractions.

Western blotting was performed using the soluble membrane fraction of LS180 or HCT116 cells with rabbit anti-OATP1B3 serum that was developed by immunizing a rabbit with the synthesized epitope peptide (LEFLNNGEHFVPSAGTD).

The substrate specificity of the PAPST1 and PAPST2 proteins was examined using the P100 membrane fraction and radiolabeled substrates.

For QTAP analysis using the plasma membrane fraction, it is important to prepare the sample with highly reproducible purity and recovery.

After separation of nuclei, ¼ of the cell lysate was kept as total homogenate for further analysis, while the remaining ¾ was used to separate out the membrane fraction.

An NADH oxidase activity assay was used to confirm that the membrane fraction containing the UCPs was in fact enriched in bacterial membranes (results not shown).