Genomic DNA was isolated from EDTA anti-coagulated blood using the membrane based QIAamp DNA extraction protocol (Qiagen, Hilden, Germany).
The membrane extraction with sorbent interface used in this research is a three-phase supported liquid membrane consisting of an aqueous (donor phase), organic solvent/nano sorbent (membrane) and aqueous (acceptor phase) system operated in direct immersion sampling mode.
Membrane and soluble protein fractions were obtained using the Proteoextract Native Membrane extraction kit (Calbiochem).
The plasma membrane fraction was extracted from cells cultured in a 10-cm dish using the Plasma Membrane Protein Extraction Kit (Biovision, Milpitas, CA), according to the manufacturer's instructions.
The plasma membrane fraction was separated from a pool of three different cryopreserved spontaneous Δ16HER2-positive tumors lysates using the Plasma Membrane Protein Extraction Kit (Biovision, Mountain View, CA) according to the manufacturer's instructions.
Bacterial pellets from 10 mL of planktonic (mid-exponential or stationary) or biofilm cultures were lysed by sonication and used for the isolation of cytosolic and membrane protein fractions using the ReadyPrep Protein Extraction Kit Membrane I (Bio-Rad Laboratories, Hercules, CA, USA).
Murine dermal fibroblasts were harvested, and then the membrane and cytosolic extracts were prepared using the Mem-PER extraction reagents according to the manufacturer's instructions (Thermo Scientific).
For Fyn - p190RhoGAP co-precipitation experiments, membrane fractions were isolated by using the Compartmental Protein Extraction Kit CNMCS (K201301-1-6, Biochain Instothee) according to the manufacturer's instructions.
Membrane and cytosolic fractions were prepared using the Compartmental Protein Extraction kit (Millipore, Temecula, CA, USA) according to the manufacturer's instructions.
The procedure for DNA extraction and purification was performed by using the silica-membrane-based spin column method.
