Sentence examples for using the megaclear kit from inspiring English sources

mRNAs and gRNAs were subsequently purified using the MEGAclear kit (Life Technologies) and resuspended in RNase-free water.

mRNAs and gRNAs were subsequently purifed using the MEGAclear kit (Life Technologies) and resuspended in RNase-free water.

After subsequent DNase I treatment, RNA was recovered using the MEGAclear Kit (Ambion).

The supernatant was cleared of small RNA molecules using the MEGAclear Kit (Applied Biosystems, Ambion, CA) and depleted of host ribosomal RNA performing two cycles of the MICROBEnrich (Applied Biosystems, Ambion, CA) protocol.

After in vitro transcription, mRNA was purified using the MEGAclear Kit (Ambion).

Products were purified using the MEGAclear kit (Life Technologies, cat. no. AM1908).

mRNAs were purified either by standard phenol chloroform extraction or using the MEGAclear Purification Kit (Ambion), and quantified by agarose-gel electrophoresis and spectrophotometry.

The resulting RNA was purified using the Ambion MegaClear kit to provide the highly purified RNA recommended for amplification (Ambion Inc., Austin TX) and then amplified using the Ambion MessageAmp II RNA amplification kit (Ambion Inc., Austin TX).

The Cas9 mRNA and the gRNAs were subsequently purified with the MEGAclear kit (Life Technologies), resuspended in RNase-free water, and quantified using NanoDrop-1000.

mRNAs were purified using either the NucAway kit (Ambion) followed by two phenol/chloroform extractions and ethanol precipitation, or the Megaclear kit (Ambion).

Sense and anti-sense single-stranded RNAs were synthesized using MEGAscript T7 kit (Ambion), purified using MEGAclear kit (Ambion) and annealed in an equimolar ratio.