Sentence examples for using the megaclear Purification from inspiring English sources

mRNAs were purified either by standard phenol chloroform extraction or using the MEGAclear Purification Kit (Ambion), and quantified by agarose-gel electrophoresis and spectrophotometry.

mRNAs and gRNAs were subsequently purifed using the MEGAclear kit (Life Technologies) and resuspended in RNase-free water.

mRNAs and gRNAs were subsequently purified using the MEGAclear kit (Life Technologies) and resuspended in RNase-free water.

After subsequent DNase I treatment, RNA was recovered using the MEGAclear Kit (Ambion).

The supernatant was cleared of small RNA molecules using the MEGAclear Kit (Applied Biosystems, Ambion, CA) and depleted of host ribosomal RNA performing two cycles of the MICROBEnrich (Applied Biosystems, Ambion, CA) protocol.

After in vitro transcription, mRNA was purified using the MEGAclear Kit (Ambion).

The resulting mRNA was purified using the MegaClear Kit (Ambion) and quantified using a NanoDrop-2000 (Thermo Scientific).

Both the Cas9 mRNA and the sgRNAs were purified using the MEGAclear kit (Life Technologies).

Products were purified using the MEGAclear kit (Life Technologies, cat. no. AM1908).

The resultant RNA was treated with DNase I (Ambion), purified using the MegaClear kit (Ambion), and precipitated with NH4OAc/EtOH.

The CRISPR guide RNA was synthesized using the MegaShortScript T7 Kit (Life Technologies) and purified using the MegaClear Kit (Life Technologies).