Sentence examples for using the mega version from inspiring English sources

Upon calculating the pair-wise evolutionary distances (Kimura, 1980), a neighbour-joining phylogenetic tree was constructed using the MEGA version 5.0.The strain was deposited at Microbial Type Culture Collection and Genebank, (MTCC 11460) and the 16S rRNA gene sequence was submitted to EMBL (HF544505) database.

The retrieved PIE1 homolog sequences were aligned using BioEdit software (http://www.mbio.ncsu.edu/BioEdit/bioedit.html), and neighbor-joining trees were generated using the MEGA version 3.1 software [41], with bootstrap values obtained from 500 replications.

Partial nucleotide sequences of 16S rRNA and rpo-β for Helicobacter and Acinetobacter and of flgK, GDP-D-mannose dehydratase and UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase for Helicobacter from cDNA sequencing, and of env gene for the retrovirus from a PCR product were aligned with the respective homologous sequences available in GenBank using the MEGA version 4 [56] software.

Phylogenetic analyses were conducted using the MEGA version 3.1 suite of programs [ 88].

Multiple sequence alignment and translation to amino acids were conducted using the MEGA version 6 program.

The phylogenetic and molecular evolutionary analyses were conducted using the MEGA version 6 [ 43].

Phylogenetic analyses were performed using the Mega software version 4.0 [ 111].

The Neighbor-Joining algorithm and the Kimura 2-Parameter model trees, with 1000 bootstrapped replicates, were built by using the MEGA package, version 5.2 [ 39].

A phylogenetic tree was generated, based on the nucleotide sequences of the supergene obtained by concatenating the seven loci for the four clinical isolates and three reference strains (M. abscessus CIP 104536 T, M. massiliense CIP 108297 T, M. bolletii CIP 108541 T), using the MEGA software, version 5.2 (http://www.megasoftware.net/) (data not shown).

Phylogenetic and molecular evolutionary analyses were conducted using the MEGA (Molecular Evolutionary Genetics Analysis) software version 4 [ 13].

The 18S rRNA gene sequences were assigned to the GenBank database in order to allow BLAST searching and alignment using the MEGA software program version 5.0 (Mega Software, Tempe, AZ, USA).