Sentence examples for using the mega primer from inspiring English sources

To understand the effects of mutation at the GGEEF signature sequence, site-directed mutagenesis using the mega primer method was utilized to generate three mutants Sebox5, Sebox6 and Sebox7 representing mutations at the second G, first E and the second E, respectively.

Cluster analysis was conducted using the MEGA 4.0 program.

Phylogenetic trees were constructed using the MEGA 3.1 [ 36] or MEGA 4 [ 37] software packages.

The unrooted tree was drawn using the MEGA package [ 86].

The phylogenetic tree was built using the MEGA 4 program.

Phylogenetic analysis was conducted using the MEGA 5.2 program [ 38].

Sequences were aligned manually using the Mega 5.2 software.

Phylogenetic tree analysis was also performed using the MEGA 5.0.

All calculations were performed using the MEGA 5.2 software.

Phylogenetic trees were constructed using the MEGA 2.1 software  [ 40].

Sequences were analyzed using the MEGA 5.0 program [ 18].