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In a phenotypic resistance assay, the degree of resistance is defined using the median inhibitory concentration (IC50).
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Using the median effect method showed that phenanthrene and GTP were antagonistic when the inhibitory levels were less than about 50%.
The results are compared with that using the median filter.
The quantitative data was assessed using the median value.
The dose response curves were obtained and then used to calculate the median inhibitory concentration (IC50).
The results are presented as percentage inhibition to the negative control (0.5% DMSO) as the following: Percentage inhibition = (1 - O D samples - O D blank / O D Vehicle - O D blank ) × 100 The median inhibitory concentrations (IC50s) were calculated using the dose response curves (n = 3).
The median inhibitory concentration (IC50) values were determined using CurveExpert 1.3 software and plotted in dose-response curves.
The median inhibitory concentration IC50 values were calculated using GraphPad Prism 5.0 software (La Jolla, CA).
The median inhibitory concentrations (IC50) of paclitaxel were calculated using Sigma Plot 8.0 software (SPSS, Inc., Chicago, IL).
The percentage inhibition of edema volume at each hour compared to the control group was calculated using the following formula: Where A= (Vt − V0 control; B= (Vt − V0 treatment The median inhibitory dose (ID50) for the inhibition of inflammation at 3rd and 4th h (peak edema) after inflammation induction was calculated.
The median inhibitory concentrations IC 50 1 ( t ) and IC 50 2 ( t ) are used to quantify drug susceptibility (Molla et al.1996) and are written as IC 50 ( t ) = I 0 + I r - I 0 t r t for 0 < t < t r, I r for t ≥ t r, (4).
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