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We therefore successfully deprived female flies of sleep over a long period via two independent methods; males were deprived of sleep using the mechanical stimulus but not the light stimulus.
Flies deprived of sleep using the mechanical stimulus have increased expression of genes involved in stress response pathways, including genes involved in the inflammatory, oxidative stress, and unfolded protein responses [34], which may account for the increase in triglycerides we observed.
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We performed an additional analysis on the flies deprived of sleep using the mechanical or the light stimulus to see if the flies were adapting to either stimulus over time.
The previous study used changes in pressure as the mechanical stimulus and there was not direct contact/linkage to the cell.
To discriminate between the effects of sleep loss and effects that were solely due to the mechanical stimulus, we deprived flies of sleep using light (see Figure 1).
Furthermore, stimulation at 60 Hz impaired phase locking of the postsynaptic activity to the mechanical stimulus.
In the case of mechanical stimuli, the cells within the subjected tissue undergo a few aspects of the mechanical stimulus, and decide to alter the tissue matrix in a pertinent manner depending upon the aptness of stimulus.
The mechanical stimulus is sufficient to start silk production.
The mechanical stimulus produced by physical activity promotes bone remodelling.
The mechanical stimulus was repeated with 10 sec intervals until the animal no longer responded to the stimulus.
Asztalos et al. [24] showed in Drosophila that the olfactory jump response to benzaldehyde can be sensitized using a mechanical stimulus.
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