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Spine length was measured using the measurement tool of AxioVision.
Lengths were measured from immunolabeled images using the measurement tool in ImageJ (NIH, Bethesda, MD).
Vertical distances in millimeters from the implant shoulder to the most apical initial point of first visible bone contact were measured for both proximal sites using the measurement tool function of the ImageJ software.
Neurite length was determined by measuring the longest process of 100 randomly selected neurites from each slide using the measurement tool in ImageJ (NIH, Bethesda, MD) as previously described.
Linear measurements of the narrowest segment of the left fourth aortic arch (between the right brachiocephalic and left common carotid arteries) were made using the measurement tool in Amira.
Spiral ganglion neuron (SGN) total neurite length was determined from digital images by measuring the longest process of 100 randomly selected neurites from each condition using the measurement tool in ImageJ (NIH, Bethesda, MD) as previously described.
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Thus validation for this tool must be completed within in the community among those health workers who will actually be using the measurement tools.
The same ROI was then superimposed onto the matching fluorescent image, and the sum of fluorescent intensity within the ROI was divided by its area to generate the mean fluorescent pixel intensity per unit area (MPI) (i.e., protein level) using the "measurements" tool in OpenLab.
Is there a cost involved to use the measurement tool?
We used the Measurement Tool to Assess Systematic Reviews (AMSTAR) [ 16] method to evaluate the methodological quality of the different papers.
Measurements from CLSM-image stacks were conducted in Imaris 7.1 using the Measurement-tool in Section-mode.
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