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The relative expression of surface antigens was evaluated using the mean fluorescence intensity (MFI).
The doubling time for each population was calculated using the mean fluorescence intensity of each timepoint over 96 hr.
To evaluate the marker expression, we determined the specific fluorescence index (SFI) using the mean fluorescence intensity (MFI).
13 18 In these studies NK cell subpopulations were defined using the level of NKp46 expression on a per-cell basis using the mean fluorescence intensity (MFI).
A relative measure of antigen expression was obtained using the mean fluorescence intensity, converted from a log to a linear scale, after subtracting the cells' self-fluorescence and the fluorescence of cells incubated with irrelevant isotype control mAbs.
A relative measure of antigen expression was obtained using the mean fluorescence intensity (MFI), converted from log to linear scale, after subtraction of the cells' autofluorescence and the fluorescence of cells incubated with irrelevant isotype control mAbs.
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Specifically, two parameters of flow cytometry were used: the mean fluorescence intensity (MFI) and the percentage of positive staining cells.
In order to compare the FM study in kidney to the two other methods used, the mean fluorescence intensity of the whole kidney, including the glomerulus (excretory compartment) and parenchyma (collecting compartment), was plotted against time in Figure 3C.
Gene-level analyses were performed using the mean normalized fluorescence values for all probes and replicates.
Using flow cytometry, the mean fluorescence intensity (MFI) values revealed that patients with stable CAD and control subjects expressed the same amount of β2-integrins on their neutrophils (349±80 and 337±104, respectively; mean MFI±SD; Fig. 1A,C).
Fluorescence for each worm was quantified by the cell profiler software (www.cellprofiler.org) using the bright‐field image to create a mask for each worm, used to measure the mean fluorescence per area after median background subtraction.
More suggestions(15)
using the mean recall
using the native fluorescence
using the inherent fluorescence
using the mean value
using the extrinsic fluorescence
using the blue fluorescence
using the portable fluorescence
using the intrinsic fluorescence
using the mean response
using the mean aggregation
using the cationic fluorescence
using the innate fluorescence
using the mean distance
using the mean method
using the mean thickness-to-length
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