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Using the mean absorbance values for each sample, corresponding concentrations were determined after plotting the standard calibration curves.
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The cut-off values for the Bio-Rad TeSeE ELISA and Idexx assays were calculated using the mean of the absorbance values of 90 confirmed BSE negative brainstem samples+ 3 standard deviations.
Growth curves were plotted using the mean ± SD of absorbance at 450 nm vs. time points.
The mean absorbance values per gene target per plate were normalized using the mean value for non-targeting siRNA (NTS) transfected cells.
The mean absorbance was used to calculate cGMP concentration from the standard curve.
The concentration of secreted VEGF was calculated using the standard curve created by plotting the mean absorbance on y-axis against the concentration on the x-axis.
The mean absorbance values were used for calculating available primary amino residues in LDL and estimating % of reactive lysyl groups modified by HNE relative to the native LDL (ϵmax = 1.92 × 10 M−1 cm−1 is the molar extinction coefficient of TNP-lys at 420 nm).
The mean absorbance data were statistically analysed using t-test to compare the average mean absorbance difference between different groups of tissues, and P<0.05 was considered significant.
The mean absorbance of honey samples was determined using the method of [2].
The mean absorbance of honey samples was determined using the method of Beretta et al. [ 7].
The relative protein level of UGDH and Sp1 was presented as the mean absorbance of each positively stained chondrocyte using NIS-elements software (Nikon, Tokyo, Japan).
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