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The positions of some cells were aligned using the maximal intensity of DAPI nuclear staining.
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The maximal intensity of the light source, providing an irradiance saturating pulse of 3000 μmol (photons) / m2 / s) was used.
To determine the depth of focus I used the following definition: a point is reconstructed at a special reconstruction distance if the intensity of the reconstructed point is not lower than the maximal intensity of its background.
This filter assigns to every pixel the maximal intensity value of its 5×5 pixel neighbourhood.
However, the maximal intensity offered by the device is the goal of the treatment intensity.
The intensities observed at the other time points were then expressed relative to the maximal intensity in each time course.
The obtained curves were fit using the sigmoidal curve in eq 1(42) 1where t is the time, t1/2 is the time to reach half-maximal ThT fluorescence, F0 is the initial fluorescence intensity, a is the amplitude of the maximal intensity, and k is the rate constant.
Z-stack images were also taken and the maximal intensity projections are shown (Fig. 4Ag i).
The maximal intensity may, in rare cases, induce transient muscular contractions.
The maximal intensity of each cell was derived from a manually selected region of interest.
The spectra were corrected for background (buffer, solvent, and each protein individually), and the maximal intensities were measured using Vinci version 1.5 (ISS Inc .. To determine dye effects, emission spectra (660 700 nm) of 25 nM Cy5-labeled hPPARα titrated with increasing concentrations of unlabeled hLXRα were recorded upon excitation at 650 nm.
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