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In order to identify the genes that were directly regulated by TFE3, we examined the gene promoters for MiTF/TFE recognition sequences using the MatInspector program (www.genomatix.de).de
Bioinformatics analysis using the MatInspector program (Genomatix) revealed potential binding sites for several beta-cell transcription factors: HNF1, HNF6, AP1, INSM1, XFD3 and PTF1, within the most conserved region of block 2 (Fig. 6B).
To determine the presence of PPARγ-RXR binding site motifs (DR1) in the PPARγ and RXR ChIP-PET genome-wide binding site data sets, we analyzed the cluster sequences using the Matinspector program, which is part of the Genomatix software suite (Genomatix, Munich).
Search for potential transcription factor binding sites was done using the MatInspector program [ 36].
Promoter sequences were examined for potential transcription factor binding sites using the MatInspector Program (www.genomatix.de).de
A search for potential TFBS in the upstream regulatory region of a particular HL gene was performed online at Genomatix using the MatInspector program [ 2, 32].
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The 1101 base pair sequences obtained from the El Dorado program then were used as the target sequences for putative transcription factor recognition site identification using the MatInspector Version 4.2 program, which yielded sites for 11 transcription factors (Genomatix Software GmbH, Munich, Germany, [ 17]).
Using the MatInspector (http://www.genomatix.de) and AliBaba 2.1 (http://www.gene-regulation.com) in silico analysis programs, we found that SP1-binding sites are commonly found in this promoter sequence (Fig. 4B).
We counted the number of times a given family appeared in each sequence using the MatInspector results.
For promoter analysis, promoter sequences (-750, +250) were retrieved from the promoter database at and screened for GATA3 consensus sites using the MatInspector promoter analysis tool [ 13].
To further study this potential regulation, we have also used the MatInspector 7.7.3 program (Genomatix Software GmbH, Munich, Germany) to analyze the putative AR binding sites on Orai1 promoter (see the appropriate section of the discussion).
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