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Recombinant clones were generated using the MARCM system (Lee and Luo, 2001).
Mutant clones were made using the MARCM system (Lee and Luo, 2001).
Stocks containing the relevant UAS-transgenes and either FRT82B or FRT40A were generated for clonal analysis using the MARCM system.
Mutant clones for tkv, put or mad were generated using the MARCM system (Lee and Luo, 2001).
Recombinant control, rk 1, and rk 4 mutant clones were generated by Flp/FRT-mediated somatic recombination using the MARCM system [ 41].
To define the step where Notch signaling is stalled in awd mutant follicle cells we over-expressed NICD or NEXT in awd mutant follicle cells by using the MARCM system.
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We used the MARCM system to misexpress the full-length otd coding sequence in the antennal lobe neuroblast clones using a Tub-Gal4 driver.
To confirm this, we used the MARCM system to generate Dg O86 clones in well-fed and starved ovaries that were deliberately damaged by vigorous pipetting.
To assess these possibilities, we used the MARCM system (Lee and Luo, 2001) to express a UAS-bazΔ 312-1464-GFP construct in baz EH747 FE clones.
To address this question, we used the MARCM system in order to label pNBs independently of neuroblast markers (Lee and Luo, 2001).
We next used the MARCM system (Lee & Luo, 1999) to create adult midgut clones from a control transgene (MARCM LacZ; Fig 2M O) or with Src42 knockdown (MARCM Src42-IR; Fig 2P R) and follow their growth at 7, 14 and 30 days after clonal induction (ACI).
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