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Clones were generated using the MARCM method [32].
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To test this possibility, we used the MARCM method to examine the effect of mutation of RnrL on clones of cells that overexpress an activated form of yorkie (yki ) (Oh and Irvine 2008).
To do this, the DN-DaPKC transgene was expressed specifically in ept,H99 mutant cells using the MARCM technique [35].
Using the MARCM technique, we have recently shown [23] that the loss of Stam impairs the migration of air sac primordium (ASP) cells; with only 16% of mutant clones detected at the ASP tip (Fig. 1C).
Mutant clones were made using the MARCM system (Lee and Luo, 2001).
Recombinant clones were generated using the MARCM system (Lee and Luo, 2001).
Recombinant clones were generated using the MARCM technique as previously described (Lee & Luo, 1999).
Stocks containing the relevant UAS-transgenes and either FRT82B or FRT40A were generated for clonal analysis using the MARCM system.
Mutant clones for tkv, put or mad were generated using the MARCM system (Lee and Luo, 2001).
Analysis of detailed axon pathfinding could then be focused to the relevant neurons using the MARCM technique (Lee and Luo, 2001).
Recombinant control, rk 1, and rk 4 mutant clones were generated by Flp/FRT-mediated somatic recombination using the MARCM system [ 41].
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