Sentence examples for using the liquid overlay from inspiring English sources

Methods: An in vitro multicellular gastric cancer spheroid model was established using the liquid overlay technique and compared with the corresponding xenografts in immunodeficient mice.

Green fluorescent protein (GFP -positive adMSCs expressinGFP -positive CD90 adMSCs117 wexpressinged from CD13L/6-TgN(ACD73EGFP)1Osb/J miCD90or the generandon of spheroids using the liquid overlay teCD117ue.

Green fluorescent protein (GFP -positive adMSCs were isolated from C57BL/6-TGFP -positive1Osb/J mice for the generadMSCsof undifferentiated and differentiated spheroids using the liquid overlay technique.

Spheroids were initiated using the liquid overlay technique.

Melanoma spheroids were prepared using the liquid overlay method (Smalley et al, 2006).

Spheroids were grown using MG-U87 cells and were formed using the liquid overlay method.

It has to be noted that the liquid overlay technique in conventional culture medium used here is not a method dedicated to CSC expansion.

We used HT29 and CT320X6 colon cancer cell lines because of their ability to form compact multicellular tumour spheroids when cultured according to the liquid overlay technique [ 6].

As an alternative to the liquid overlay, agar overlays were also investigated.

Three-dimensional multicellular spheroids were prepared by the liquid overlay technique [ 6].

Three-dimensional multicellular spheroids were prepared by the liquid overlay technique as previously described (Dangles-Marie et al, 2007).