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The quantitative assay of the antimicrobial activity against planktonic microbial strains was performed using the liquid medium microdilution method, in 96-multiwell plates, in order to establish the minimal inhibitory concentration (MIC).
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The porous hydroxyapatite/collagen (HAp/Col = 80/20 in weight ratio) composite with unique three-dimensional pore structures was fabricated by the control of ice crystal growth using the liquid N2 as a cooling medium and subsequent freeze-dry process, and the pore structure and mechanical properties were evaluated by SEM and compression tests.
The assay was performed by using the EndoCult Liquid Medium Kit® (StemCell Technologies, Vancouver, BC, Canada) using the manufacturer's protocol.
After 7 days of SmF culture using the optimal liquid medium (w/v) of yeast extract 1.48%, coffee pulp 1.94%, wheat bran 0.38%, sawdust 1.22% and urea 4.98% (Eqn 6; Table 2; Table 4), LiP from S. cinnamomensis reached a peak of 0.1450 U/mL, which was approximately 8.2 times activity of the initial average value of 0.0176 U/mL (Table 1) and higher than the expected peak of 0.1339 U/mL (Table 2).
Zeta potential value was calculated on the basis of electrophoretic mobility of solid particles in the liquid medium using the Smoluchowski equation [32].
The liquid medium used in the fluidic chamber was silicone oil (Fisher Scientific, S159-500).
After the desired incubation time, the liquid medium was removed using a vacuum and the planktonic cells were removed by washing the well once with MilliQ water.
2) Seed medium: the liquid medium of DI1 was used as the seed medium.
The liquid medium dilution technique is used more frequently than the agar diffusion method when evaluating the antimicrobial activity.
Without using a liquid medium, iCVD enabled the coating of fine particles down to the nanoscale without particle agglomeration.
The influence of pH and aeration on AMS production was tested in liquid medium using the chemically defined medium containing glucose [ 32].
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