Sentence examples for using the liquid isolation from inspiring English sources
Genomic DNA from the BGM clones was prepared using the liquid isolation method [ 12].
Toss the solids and use the liquid.
Total RNA was extracted from tuber tissue powdered in liquid nitrogen, using the Plant RNA Isolation Kit from Invitrogen (Karlsruhe, Germany) following the supplier's protocol.
Total RNA was extracted from the adults of D. viviparus (under liquid nitrogen, employing a sterile mortar and pestle) using the TriPure isolation reagent® (Roche Molecular Biochemicals).
The volatile compounds of the oil were collected in diethyl ether using liquid-liquid isolation.
All these samples were ground in liquid nitrogen using a sterile mortar and pestle, and then the RNA was isolated using the mirVana microRNA isolation kit according to manufacture's instructions (Ambion, Applied Biosystems).
Frozen cells were ground under liquid nitrogen and total RNA isolated from the cell powder using the mirVana RNA isolation kit (Ambion).
Total RNA from frozen biopsies stored in liquid nitrogen was isolated following the procedure indicated by the manufacturer using the mirVana RNA isolation kit (Ambion-Life Technologies, Grand Island, NY, USA).
Soil samples (∼0.30 × g), abdominal liquid, and abdominal and skin swabs were extracted using the PowerSoil DNA isolation kit (Mo Bio Laboratories, Carlsbad, CA, USA) at the University of Colorado, Boulder.
Samples (biological replicates) were immediately flash frozen in liquid nitrogen, stored at -80°C, ground in liquid nitrogen and total RNA was isolated using the protocol of TriPure Isolation Reagent (Roche Applied Science, http://www.roche-applied-science.com).com
