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Fermentability tests using the liquid fraction showed a higher number of colony-forming units (CFU) and higher xylose consumption rates when treating the medium with laccase.
The effect of laccase treatment on phenolic compounds in corn stover prehydrolysate was evaluated using the liquid fraction obtained from pretreatment.
Although, fed-batch adaptation of yeast using the liquid fraction did not contribute to fermentation in batch SSF of spruce slurry, reducing the inhibitors by diluting the liquid fraction in anaerobic fermentation significantly improved the fermentation performance.
It has been shown that prior to SSF, cultivating the cells in fed-batch mode using the liquid fraction derived from pretreatment improved tolerance towards inhibitors and ethanol productivity in an SSF process using pretreated spruce of relatively low WIS content of 8% (w/w) [ 26].
To improve cell viability and xylose consumption, the liquid fraction was diluted to the equivalent of 12% DM content and inoculated with 5 g/L of evolved S. cerevisiae KE6-12, because of the best growth at this inoculum size when using the liquid fraction corresponding to 14% DM.
However, when the cells were cultivated in a continuous mode using the liquid fraction and fed to the SSF process with substrate feed, the overall ethanol yield was significantly increased to 44%and53%3% of the theoretical maximum based on available hexose sugars with no enzyme feed and in combination with enzyme feed, respectively.
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It is therefore not unreasonable to use the liquid fraction rich in organic matter to produce biogas.
Vaccine preparation consisted of reconstituting the lyophilized antigen using the liquid Adjuvant System fraction, stored in separate vials, to a final volume of 0.5 mL.
Here we have evaluated an alternative use for the liquid fraction of the major forage species, Lolium perenne, to examine if it can be used as a bio-stimulant for use on field and horticultural crops.
Since many of the inhibitors commonly found in spruce hydrolysate act synergistically [ 31], the use of the liquid fraction of the pre-treated biomass for the evolution was expected to lead to a greater resistance of the yeast towards this cocktail of inhibitors.
Laccase was used to detoxify the liquid fraction before fermentation tests.
More suggestions(15)
using the liquid medium
using the liquid injection
using the liquid retention
using the liquid quenching
using the liquid pulse
using the liquid sputtering
using the liquid overlay
using the liquid Resin
using the liquid biopsy
using the liquid chromatography/quadrupole
using the liquid scintillation
using the liquid nitrogen
using the liquid jet
using the liquid drop
using the liquid curing
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