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After that, screening should be done every year with the regular Pap test or every two years using the liquid Pap until age 30.
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All three studies used the liquid Pap test sample; however, they examined the cellular component of the Pap test for either DNA mutations that were known to be present in tumors from the same patient [ 42], or for MS profiles of cytospins [ 41] or laser capture microdissected cells from ThinPrep slides [ 22].
A newer version, the liquid Pap, in which the cervical cells are suspended in liquid first and then viewed in a single debris-free layer, is considered more likely to produce an accurate assessment of a cancer risk.
Use the liquid.
Toss the solids and use the liquid.
Cytology specimens were obtained with a moistened Dacron swab (PurFybr, Munster IN) and processed using the SurePath Liquid-based Pap Test system [4], [5].
Clinical specimens were collected per routine procedures using the BD SurePathTM liquid-based Pap test.
The amount of free glucose remaining in the culture supernatants was measured using the Glucose GOD-PAP Liquid Stable Mono-reagent kit (LaborLab Laboratories Ltd. Guarulhos, São Paulo, Brazil), according to the manufacturer's instructions.
The amount of free glucose remaining in the medium was measured using the Glucose GOD-PAP Liquid Stable Mono-reagent kit (LaborLab Laboratories Ltd. Guarulhos, São Paulo, Brazil), according to the manufacturer's instructions.
Glucose and xylose concentrations of the supernatants were measured using the Glucose GOD-PAP Liquid Stable Mono-reagent enzymatic kit from LaborLab Laboratories Ltd. (Guarulhos, SP, Brazil) and the D-xylose assay kit from Megazyme.
Plasma triglycerides were determined using the GPO PAP kit from Instruchemie (Delfzijl, Netherlands).
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