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The mean PCR efficiency (E) for every primer pair was calculated using the linregPCR software [ 119].
The PCR efficiency from the exponential phase (E) was calculated for each individual amplification plot using the LinRegPCR software [ 90].
The efficiency of each of the 192 target gene PCR primer pairs was calculated using the LinRegPCR software [ 22].
PCR amplification efficiency was determined by applying linear regression analysis to the exponential phase of the amplification curve of each PCR reaction using the LinRegPCR software (79).
The efficiencies of PCR of ω-2 and ω-5 primers were estimated as 101·8 and 100·7 %, respectively, using the LinRegPCR software (Ramakers et al., 2003).
Amplification efficiency for each primer pair was calculated using the LinRegPCR software application [ 24] and the mean efficiency values for each primer were added to Additional file 1.
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The method used to calculate the relative amount of cDNA was described by Liu and Saint [30], and the relative efficiencies for individual amplification were calculated using the LinRegPCR program [31].
PCR efficiencies were calculated using the LinRegPCR program http://www.gene-quantification.de/download.html linregpcr.html linregpcr
PCR amplification efficiency was calculated using the LinRegPCR program [ 67].
The data were analyzed with an R above 0.998 using the LinRegPCR program [ 42].
The data was analysed with an R >0.998 using the LinRegPCR program [ 75].
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