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Primer efficiencies were calculated as the average of individual well efficiencies determined by linear regression of amplification curves using the LinRegPCR software [ 72].
Relative quantification and statistical analysis were performed using the LinRegPCR software version 11.1 [ 35], which is using the linear regression analysis to calculate the starting concentrations of mRNA's and individual PCR efficiencies for each sample.
PCR amplification efficiency was determined by applying linear regression analysis to the exponential phase of the amplification curve of each PCR reaction using the LinRegPCR software (79).
The method used to calculate the relative amount of cDNA was described by Liu and Saint [30], and the relative efficiencies for individual amplification were calculated using the LinRegPCR program [31].
PCR efficiencies were calculated using the LinRegPCR program http://www.gene-quantification.de/download.html linregpcr.html linregpcr
PCR amplification efficiency was calculated using the LinRegPCR program [ 67].
The mean PCR efficiency (E) for every primer pair was calculated using the linregPCR software [ 119].
The data were analyzed with an R above 0.998 using the LinRegPCR program [ 42].
The data was analysed with an R >0.998 using the LinRegPCR program [ 75].
The PCR efficiency of each primer set was evaluated using the LinRegPCR program [ 68].
The data was analyzed with an R2 above 0.998 using the LinRegPCR program [ 52].
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