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The method used to calculate the relative amount of cDNA was described by Liu and Saint [30], and the relative efficiencies for individual amplification were calculated using the LinRegPCR program [31].
PCR amplification efficiency was calculated using the LinRegPCR program [ 67].
The data was analysed with an R >0.998 using the LinRegPCR program [ 75].
The PCR efficiency of each primer set was evaluated using the LinRegPCR program [ 68].
The data were analyzed with an R above 0.998 using the LinRegPCR program [ 42].
PCR efficiencies were calculated using the LinRegPCR program http://www.gene-quantification.de/download.html linregpcr.html linregpcr
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PCR efficiency of each run was calculated using the LinRegPCR programme http://LinRegPCR.nl[ 66].
The mean PCR efficiency (E) for every primer pair was calculated using the linregPCR software [ 119].
The efficiency of each of the 192 target gene PCR primer pairs was calculated using the LinRegPCR software [ 22].
The PCR efficiency from the exponential phase (E) was calculated for each individual amplification plot using the LinRegPCR software [ 90].
The efficiencies of PCR of ω-2 and ω-5 primers were estimated as 101·8 and 100·7 %, respectively, using the LinRegPCR software (Ramakers et al., 2003).
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