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The large-scale reaction worked, and Cytel is now using the linked enzymes to make up to 15 kilograms of sialyl lactose, a component of breast milk that the company hopes to sell as an additive for infant formula.
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A total of 1397 groundnut samples collected from farm homesteads, local markets, warehouses and shops in 2008 and 2009 were analyzed for AFB1 contamination using the enzyme linked immunosorbent assay (ELISA), and A. Aspergilli population densities in 1053 soil samples collected from the same sites were estimated using serial dilutions plated on A. Aspergilli medium.
Serum PCT concentrations were determined using the enzyme linked immunosorbent assay (ELISA).
Quantitative D-dimer levels were obtained using the enzyme linked fluorescent assay (ELFA) on a mini VIDAS system (bio-Merieux).
The concentration of D-Dimers in platelet poor plasma was determined using the enzyme linked fluorescent assay on a mini VIDAS system (bio-Merieux, Paris, France).
Due to the non-specific clinical manifestation, the diagnosis of sporadic norovirus infection can be made by detection of viral nucleic acid using reverse transcription-polymerase chain reaction (RT-PCR) assays or by detection of viral antigens in faeces using the enzyme linked immunosorbent assays (ELISA) or the immunochromatographic (ICG) tests [ 9– 11].
IL-6 and TNF-α were measured using the DuoSet enzyme linked immunoabsorbent assay (ELISA) kit (R&D systems) following the manufacturer's instructions.
Serum samples were screened for HAV and HEV specific IgG antibodies by using the commercial enzyme linked immunosorbent assays (ELISA): bioELISA HAV IgG and bioELISA HEV IgG (Biokit, Spain) according to manufacturer's instructions.
The amount of insulin released in vivo was quantified using the Enzyme Linked Immunosorbent Assay (ELISA), and its bioactivity was determined by its ability to reduce the blood glucose levels in diabetic rats.
The presence of Toxoplasma-specific IgG, IgM and IgA antibodies was investigated in serum and colostrum samples from puerperal women by using the Enzyme Linked Immunosorbent Assay (ELISA) and Immunobloting assays.
Secretion of CXCL8 was assayed using the Duoset enzyme-linked immunosorbent assay (ELISA R&D Systemsms).
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