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Elemental concentration profiles across both stacking faults and slip bands have been measured in a FEG TEM in nano-probe using the line scanning mode and EDX.
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For the holes, structures were measured using the line scan tool of the software, whereas the mesoscopic GaAs structures were analyzed using the grain finding function with the statistical analysis module.
Cell alignment (Fig. 1D) and filopodium angle measurements (Fig. 4C and E) were performed manually using the line scan tool.
We used the line scan program (normalize + align analysis) on a monthly basis for four visits, until clinical healing of the tubercles was achieved.
Briefly, we drew lines along the axon, starting at the injury site or soma, then used the line scan tool to measure the average fluorescence intensity of 8 pixels (∼0.7 μm) surrounding the lines.
The very fast molecular dynamics observed with "freely" diffusing molecules in the cytoplasm are better visualized by either single-point FCS or by RICS, whereas the medium-speed (millisecond) dynamics seen for molecules interacting with adhesions are better detected using the line- or circle-scan correlation methods as well as with high-speed, high-sensitivity cameras exploiting TIRF methodology.
All scans were reconstructed using the line of response reconstruction algorithm from Philips Medical Systems (4 iterations × 10 subsets).
For the CFP and YFP co-localization experiments, simultaneous imaging was conducted using the line-sequential multitrack scanning mode of the microscope to exclude the possibility of crosstalk between the fluorophores.
The white solid line in Fig. 2H indicates the 600 pixel segment used for the line scan analysis in Fig. 2I.
An argon laser (488 nm) was used in the line scan mode to provide a linear stimulus moving at the speed of 4 μs per pixel.
We tested this concept by Ca2+ imaging experiments using confocal line scanning fluorescence microscopy.
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