Sentence examples for using the lightcycler technology from inspiring English sources

The relative quantity of the human APPswe and the human PS1M146V transgenes was determined using the LightCycler technology (Roche Applied Science).

mRNA expression analyses of the genes coding for Synaptophysin (Syp), Disk large homolog 4 (Dlgh4), and Drebrin (Dbn1) were performed using the LightCycler technology (Roche Applied Science).

Of notice, the concentration of MgCl2 in real-time PCR using the LightCycler technology is higher than that used in conventional PCR.

Design and methods: We used the LightCycler technology for simultaneous detection of the H63D and C282Y mutations of the HFE gene in patients with a higher prevalence for hemochromatosis.

Assays were performed with 2.0 LightCycler technology using the LightCycler FastStar DNA Master PLUS SYBR Green I kit (cat. no. 03515885001; Roche Applied Science) as recommended by the manufacturers.

For the DNA methylation tests, quantitative methylation-specific PCR (QMSP) using TaqMan-based technologies was performed using the Lightcycler LC480 real-time PCR system (Roche Applied Science, Penzberg, Germany).

Analysis with SYBR green technology were realized by using the LightCycler FastStart DNA Master Mix SYBR Green I kit (Roche Diagnostics, Meylan, France).

Expression was assessed by real-time PCR using the LightCycler® 480 Real-Time PCR System (Roche Diagnostics, Barcelona, Spain) and TaqMan® technology suitable for relative gene expression quantification.

Gene expression was assessed by real-time PCR using the LightCycler® 480 Real-Time PCR System (Roche Diagnostics, Barcelona, Spain) and TaqMan technology suitable for relative gene expression quantification following manufacturer's protocol.

HES1 transcripts were amplified using the LightCycler Taqman DNA Master Kit and a LightCycler 480 PCR machine (Roche Applied Science).

mRNA levels were calculated using the LightCycler 480 software.