Sentence examples for using the lightcycler system from inspiring English sources

To identify these mycobacterial isolates in primary blood culture broths, we developed a multiple hybridization probe-based real-time PCR assay using the LightCycler system.

Study design: Firstly, we assessed the SYBR green real-time quantitative PCR for HIV-1 proviral DNA load detection determining the specificity and sensitivity of the assay using the LightCycler system.

CYP1A1 mRNA expression, normalized to β2-microglobulin, was determined using the LightCycler System (Roche Diagnostics, France).

Quantitative PCR was performed using the Lightcycler system (Roche, Germany) utilizing SYBR green to detect amplification.

Triplicate independent quantitative real-time PCR were performed using the LightCycler System (Roche diagnostics, GmbH Mannheim, Germany).

Real-time PCR was performed in duplicate for each RNA preparation using the LightCycler system (Roche) with an appropriate dilution of cDNA as a template.

Real-time monitoring of polymerase chain reaction (PCR) reaction was done using the LightCycler™ system (Roche Molecular Systems, Indianapolis, USA) and the SYBR Green I dye, which binds preferentially to double stranded DNA.

The aim of this study was to evaluate the feasibility of detecting Staphylococcus aureus and coagulase-negative staphylococci (CoNS) and of identifying methicillin resistance directly in positive BACTEC blood culture bottles using the LightCycler® system.

HRM assays were developed to allow screening of the ryanodine receptor gene (RYR1) for potential mutations causing malignant hyperthermia (MH) and/or central core disease (CCD) using the LightCycler 480 System.

RT-PCR was performed by using the Lightcycler 480 System (Roche Diagnostics, Basel, Switzerland).

Quantitative real-time PCR (qPCR) amplification of cDNA and analysis was carried out using the LightCycler 480 System (Roche), with LightCycler software version 1.5.