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The reactions were performed on the LightCycler platform (Roche Diagnostics GmbH, Mannheim, Germany) and results were calculated using the LightCycler software and expressed in copies/mL.
Data analyses were performed using the LightCycler software (Roche) and normalized with respect to invariant S26 mRNA levels.
* Annealing temperature; **Product Data analysis was done by using the LightCycler software version 4.0.
Fluorescence data obtained were analysed using the LightCycler software (software version 3.5, Roche Diagnostics).
Absolute quantification of plasma DNA was achieved using the Lightcycler software (version 3.5.2; Roche, Lewes, UK).
The data obtained were analyzed using the Lightcycler software provided by the manufacturer.
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mRNA levels were calculated using the LightCycler 480 software.
The results were analyzed using the LightCycler 480 software release 1.5.0 (Roche).
Data analysis was performed using the LightCycler Nano software version 1.0 (Roche).
The melting peaks were calculated using the LightCycler 480 Software Release 1.5.0SP3.
In each PCR run, a standard curve was generated using serial dilutions ranging from 10 to 108 copies of the intergenic spacer region, and the results were calculated using the LightCycler 5.32 software (LC-Run version 5.32, Roche).
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