Sentence examples for using the lightcycler detection from inspiring English sources

The relative expression levels of target genes in the duodenum and liver were assessed by Q-RT-PCR using the LightCycler detection system (Roche, Rotkreuz, Switzerland).

The amount of human S100P transcript in different tissues was assessed by quantitative real-time RT-PCR using the Lightcycler detection system (Roche, Rotkreuz, Switzerland).

The relative expression levels of the target genes in the stomach wall were assessed by QRT-PCR using the LightCycler detection system (Roche, Rotkreuz, Switzerland).

One microliter of the cDNA was used as the template for quantitative PCR (SYBR Green #K0221, Fermentas), which was performed using the Lightcycler Detection System (Roche, Basel, Switzerland), according to the manufacturer's instructions.

The relative expression levels of target genes in mouse liver, skeletal muscle and heart were assessed by quantitative real-time RT-PCR using the Lightcycler detection system (Roche, Rotkreuz, Switzerland).

HSV-1 and HSV-2 shedding were detected qualitatively by PCR using the LightCycler HSV 1/2 Detection Kit (Roche Diagnostics, Mannheim, Germany).

To validate the expression of viral miRNAs in SGIV-infected samples, real-time quantitative PCR based on TaqMan MicroRNA assay was performed using the LightCycler® 480 Detection System (Roche, Basel, Switzerland) using TaqMan® microRNA Assays kit (Applied Biosystems).

APOE genotype, a genetic risk factor for Alzheimer disease, will be performed after DNA isolation from 10 ml EDTA blood by using the LightCycler APOE mutation detection method (Roche Diagnostics GmbH) and coded as APOE ε4 or no ε4.

Real-time PCR was carried out using the LightCycler Real-Time detection system (Roche Diagnostics, Mannheim, Germany), according to the manufacturer's protocol, for 50 cycles of 10 s at 95°C, 10 s at 60°C, and 10 s at 72°C.

Real-time PCR was done using the LightCycler system with SYBR Green detection and Tm analysis.

Chicken and duck ND5 and cytb gene abundances were determined by qPCR using the LightCycler 480 real-time PCR detection system (Roche480, USA).