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Mice infected with GFP-labelled bacteria were analysed using the Leica macrofluo instrument (Wetzlar, Germany) equipped with the Leica application suite 3.1.0 software.
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Images were obtained in a high-resolution Leica DMI6000B microscope using the Leica LAS-X software.
Images were collected at room temperature on a Leica DM6000 FS microscope with a × 10 long working distance objective lens and a Leica DFC 345 FX camera using the Leica Application Suite software.
All images were acquired using the Leica SP8 X WLL Confocal microscope using the 63x glycerol immersion lens, NA = 1.44.
Napsin A was stained using the Leica Bondmax autostainer (Leica, Buffalo, IL).
Then samples were scanned using the Leica SPE laser scanning confocal microscope (Leica Microsystems, Heidelberg, Germany).
Images were processed using the Leica application suite (version 2.8.1).
Immunofluorescence was detected using the Leica DMI 6000B fluorescence microscope with a Leica DFC 350FX fluorescence camera (Leica).
Image acquisition and processing were carried out using the Leica Confocal Software (Leica Lasertechnik).
Images were processed using the Leica Confocal Software (Leica Microsystems) and Adobe Photoshop 7.0.
The labeled cells were subsequently viewed with a fluorescent (Leica DMI400B) microscope (Leica Microsystems, Heerbrugg, Switzerland) at 350 555 nm; images were captured using the Leica application suite Software.
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