Sentence examples for using the later described from inspiring English sources

Methylation-specific PCR was performed using the later described primer pairs in a volume of 50 μl containing 1 × PCR buffer, 0.2 mM dNTP, 1.5 mM MgCl2, 50 ng bisulfite-modified genomic DNA, 10 pmol each sense primer and each antisense primer, and 0.4 U Platinum Taq DNA polymerase (Gibco BRL, Gaithersburg, MD, USA).

However, as these are heterologous assays based on overexpression of proteins, it is absolutely necessary to present co-immunoprecipitation data using the later described bZIP63 GENE construct (and not 35S ox lines) and αAKIN10 western from wt and akin10 mutant plants.

When measuring on 2D we used the method later described by Hansson et al [ 14].

He then defused the uprising by deploying his soldiers around the area and persuading the ringleaders to back down — using what Carter later described as "a very subtle use of coercion and negotiation".

The 589 subjects in the exposed group were initially matched to a pool of 7390 subjects in the unexposed group using propensity scores (within a range of ±0.005) computed using the covariates described later.

This pulsed surface preparation protocol is used for all later described experiments unless otherwise noted.

RNA was isolated at a later date using the protocol described above or the adapted protocol associated with the QIAamp RNA Blood Mini Kit for use in the QIAcube (Qiagen, Valencia, CA).

Serologic profiling of each patient was performed by using the standard immunoassays described later.

However, the volume of necrotic tissue was determined using the segmentation analysis described later in the text rather than using linear measurements.

Seeds that failed to establish were replaced with a second set of seedlings a month later using the same methods described above.

Bringing together all the above parameters, the Functional Coherence index (FC) is calculated using the empirical formula described later (Equation 2), where a coherence index of 1 indicates functional coherence of the parent node and 0 indicates that functionally diverse child nodes have been merged to form the parent node.